home
***
CD-ROM
|
disk
|
FTP
|
other
***
search
/
Shareware Overload Trio 2
/
Shareware Overload Trio Volume 2 (Chestnut CD-ROM).ISO
/
dir26
/
med9410p.zip
/
M94A3211.TXT
< prev
next >
Wrap
Text File
|
1994-10-25
|
2KB
|
38 lines
Document 3211
DOCN M94A3211
TI Evidence for retroviral insertional mutagenesis in a subset of
HIV-associated lymphomas.
DT 9412
AU Herndier B; Shiramizu B; McGrath M; University of California, San
Francisco 94110.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):128 (abstract no. PA0130). Unique
Identifier : AIDSLINE ICA10/94369364
AB OBJECTIVE: To characterize on the molecular level a new class of
HIV-associated lymphomas where the retrovirus HIV has inserted
non-randomly into the tumor genomic DNA material. METHODS: 1) frozen
section and paraffin section immunohistochemistry; 2) Southern blot
analysis of inverse PCR products; 3) direct PCR sequencing; 4) mRNA
analysis (Northern blots, cDNA-PCR). Abbreviations: Mo-macrophage;
AILD-angioimmunoblastic lymphadenopathy. RESULTS: Inverse PCR (iPCR)
uses the ability of restriction digests (Sau3a), circularization, and
long terminal repeat primers to capture flanking sequences of the
inserted retrovirus. Direct sequencing showed insertion of retroviral
LTR sequence in exon Z of the fur fps/fes gene complex. The PCR products
were subsequently probed with LTR and fur oligo's on Southern blots.
TABULAR DATA, SEE ABSTRACT VOLUME. CONCLUSIONS: Fur fps/fes is a
proto-oncogene complex (tyrosine kinase) activated in some myeloid
tumors. The non-random (4 cases) clonal integration of HIV near this
locus and subsequent differential activation of mRNA compared to control
tissue (1 case) indicates that these lymphomas may have undergone
retroviral insertional mutagenesis.
DE Acquired Immunodeficiency Syndrome/*COMPLICATIONS Hodgkin's
Disease/ETIOLOGY/GENETICS/MICROBIOLOGY Human HIV/*GENETICS HIV Long
Terminal Repeat Lymphoma/ETIOLOGY/GENETICS/*MICROBIOLOGY *Mutagenesis,
Insertional Oligonucleotide Probes Polymerase Chain Reaction/METHODS
Proto-Oncogenes Restriction Mapping Retroviridae/GENETICS/*ISOLATION &
PURIF RNA, Messenger/ANALYSIS Virus Integration MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).